High efficiency DNA delivery into swine oocytes and embryos by electronic pulse delivery (EPD)
نویسندگان
چکیده
منابع مشابه
Direct DNA delivery into zebrafish embryos employing tissue culture techniques.
The production of transfected fish embryos requires expertise in injecting the fertilized eggs and/or expensive equipment for electroporation or microprojectiles. This article demonstrates that by exposure to DNA constructs conjugated with transfecting reagents dechorionated Danio rerio embryos are capable of acquiring extracellular DNA and expressing reporter genes. Embryos incubated with pCMV...
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Mouse preimplantation development is characterized by three major transitions and two lineage segregations. Each transition or lineage segregation entails pronounced changes in the pattern of gene expression. Thus, research into the function of genes with obvious changes in expression pattern will shed light on the molecular basis of preimplantation development. We have described a simplified a...
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The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross t...
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The zebrafish is widely used for functional studies of vertebrate genes. It is accessible to manipulations during all stages of embryogenesis because the embryo develops externally and is optically transparent. However, functional studies conducted on the zebrafish have been generally limited to the earliest phase of activity of the gene of interest, which is a limitation in studies of genes th...
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This letter is on behalf of a number of institutions that participate in the debit card industry in this country. We have been working together to address the debit interchange implementation challenges surrounding the recent Dodd-Frank Wall Street Reform and Consumer Protection Act (the "Act") amendments to the Electronic Fund Transfer Act ("EFT A"). As you know, section 920 of the EFT A, as a...
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ژورنال
عنوان ژورنال: Cell Research
سال: 1997
ISSN: 1001-0602,1748-7838
DOI: 10.1038/cr.1997.5